when annealing should the brass be virgin? or should it be shot once? or does it make any difference?
New brass is of no use for anything serious. It is in no way consistent until formed to what you will make of it (fire formed & sized). Early annealing serves to aid in consistent forming, and preventing of splits/cracks, -if forming a new cartridge.
I try to steer clear of extremes, as no extreme is free.
If I'm beginning with a new batch of Lapua, I'll fire form several times and then complete load development before annealing. Afterwards, I anneal as needed given a trend with pre-seating force measurements.
This is how I manage neck tension that's matching what I've load developed with.
I could decide up front to anneal every time, develop with that, and it might work out alright. However, I partial neck size minimum amount and prefer more bullet grip (tension) than constantly annealed necks would provide.
While I'm sure loads will always benefit from low deviation in neck tension, annealing does reduce tension, and I know that not all loads prefer this.
If I were loading for 6PPC or 30br (underbores), which prefer high starting pressures, I doubt that I would ever anneal them. If I were loading for an overbore, like 6.5-284, I might choose to anneal every time.