Brass usually starts getting hard after about four or five times after being fired and resized.I really started to notice this more after I got my electric trimmer.Newer brass was easy to trim,but after four or five times it was getting harder and I was getting chatter when I was trimming.Another thing I noticed,my runout would also start to get higher too.I think the hardened brass makes seating a little more difficult and may cause runout to be higher.If I'm working on hunting loads,I like to use once fired brass.If I'm working on a new test load,I like to use once or twice fired brass.I don't want brass hardness to be a factor in neck tension and I also check the runout.It has really brought to my attention the importance of keeping your brass separated not only by the brand but also by the number of times it's been fired.It takes a little more time,but every case I load,with a Sharpie I write on the case,bullet weight,type and powder charge,and number of times fired.Later if I'm going through four or five ammo boxes that have fired cases in them,I can segregate them out by the number of times fired when I get ready to do some case prep.