The reason for a ladder test is to find the harmonic node in your barrel where the bullet is leaving the muzzle at the same time that the harmonic vibration is closest to the chamber. Just as a guitar string vibrates as you pluck it, your barrel vibrates back and forth, causing the muzzle to move in an "epicylic" pattern.
As stated above, load your rounds with an increasing charge weight. I would fire them one at each load, carefully watching for pressure signs. Fire each powder charge at it's own target, keeping the same point of aim. Shoot one of each: IE 45g, 45.5, 46, 46.5 (whatever your steps are) then go back down: 46.5, 46, 45.5 45.
Now, look at your target, you should see a "node" where the vertical spacing of the shots the least, usually you will see this in a 1G to 1 1/2G spread. Do NOT focus on the groups, focus on the vertical dispersion. Take this range of charge weight that gives you the least vertical dispersion and concentrate on the charge weight in the middle. You can tweek it from there.You now have your powder charge identified. Now focus on seating depth and overall group size, loading three to five round groups and adjusting your seating depth to find a tight group. Remember to only change one factor at a time.
Perform this at 300yards if possible, 200 is OK. At 100Y you typically wont see enough change to make much sense.